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prime script rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script rt reagent kit
    Prime Script Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+kit/PrimeScript+RT+Reagent+Kit/pmc13101298-80-6-11
    Average 99 stars, based on 88323 article reviews
    prime script rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Novel Hsp90 inhibitor JD-02 inhibits HSV-1 infection via the Raf/MEK/ERK signaling pathway
    Article Snippet: Finally, fluorescence images were acquired using a Zeiss LSM510 Meta confocal system (Zeiss GmbH). .. Total RNA was extracted from the indicated treated cultured cells using TRIzol Reagent (TRIzol; cat. no. DP424; Tiangen Biotech Co., Ltd.), and a PrimeScript RT kit (cat. no. RR036A-1; Takara Bio, Inc.) was used for cDNA synthesis according to the manufacturer's instructions. .. The RT-qPCR assay was performed in a CFX96 Touch-Real-Time PCR detection system (Bio-Rad Laboratories, Inc.) using the TB Green Premix Ex Taq II kit (cat. no. RR820; Takara Bio, Inc.) according to the manufacturer's instructions ( ).

    cDNA Synthesis:

    Article Title: Novel Hsp90 inhibitor JD-02 inhibits HSV-1 infection via the Raf/MEK/ERK signaling pathway
    Article Snippet: Finally, fluorescence images were acquired using a Zeiss LSM510 Meta confocal system (Zeiss GmbH). .. Total RNA was extracted from the indicated treated cultured cells using TRIzol Reagent (TRIzol; cat. no. DP424; Tiangen Biotech Co., Ltd.), and a PrimeScript RT kit (cat. no. RR036A-1; Takara Bio, Inc.) was used for cDNA synthesis according to the manufacturer's instructions. .. The RT-qPCR assay was performed in a CFX96 Touch-Real-Time PCR detection system (Bio-Rad Laboratories, Inc.) using the TB Green Premix Ex Taq II kit (cat. no. RR820; Takara Bio, Inc.) according to the manufacturer's instructions ( ).

    Article Title: Nrf2 Protects Against Lipoteichoic Acid-Induced Acute Lung Injury By Suppressing SLC24A2-Mediated Ferroptosis.
    Article Snippet: .. RT-qPCR Total RNA was extracted from lung tissue using TRIzon reagent (CWBIO, CW0580). cDNA synthesis was performed with the PrimeScript RT kit (Takara, RR047A), and quantitative PCR was AR TIC LE IN PR ES S carried out using SYBR Green Master Mix (Yeasen, 11198ES08) on a LightCycler 480 system (Roche). ..

    Reverse Transcription:

    Article Title: miR-1307-5p mediates endothelial dysfunction and inflammation induced by acute coronary syndrome by targeting PIM3.
    Article Snippet: To regulate AR TIC LE IN PR ES S miR-1307-5p and PIM3 expression in HUVECs, negative control (NC) mimics, miR-1307-5p mimics, and PIM3 overexpression (oe PIM3), NC overexpression (oe NC) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. Reverse transcription quantitative real-time PCR (RT-qPCR) Total RNA was isolated from serum and cells by means of TRIzol (Invitrogen, USA). complementary DNA was synthesized from RNA with the PrimeScript RT Kit (TaKaRa, Japan). miR-1307-5p and PIM3 levels were quantified using SYBR Green I Master Mix Reagent (Invitrogen, USA) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). miR-1307-5p/PIM3 levels were normalized using U6/ glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls and calculated by the 2 −ΔΔCt method. ..

    Article Title: Gut microbiota dysbiosis drives depression-like behavior in adolescent rats via lysine-regulated mTOR autophagy pathway.
    Article Snippet: 2.5 Real-time quantitative PCR (RT-qPCR) Total RNA was extracted from rat brain tissues using the RNAsimple Total RNA Kit (TIANGEN, DP419) according to the manufacturer's protocol. .. Approximately 1 μg of total RNA was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript RT kit (Takara, RR047A). .. Relative mRNA expression was quantified by RT-qPCR using TB Green Premix (Takara, RR820A) under the following conditions: initial denaturation at 95 °C for 30 s, followed AR TI CL E IN P RE SS by 40 cycles of 95 °C for 5s and 60 °C for 30 s. Autophagy-related gene expression in rat brain tissue was analyzed using a targeted RT-qPCR array (Qiagen, 249950).

    Article Title: Mechanistic insights into 18β-glycyrrhetinic acid-induced apoptosis in SCC-9 cells revealed by TMT proteomics and network pharmacology
    Article Snippet: .. SCC-9 cells were treated with 90 μM 18β-GRA (high group) or negative control (0.05% DMSO) for 48 h. Total RNA was then extracted using TRIzol Reagent (Invitrogen, Cat. No. 15596026, USA) and reverse-transcribed into cDNA using the PrimeScript RT Kit (Takara, Cat. No. RR047A, Japan). qPCR was performed with TB Green Premix Ex Taq II (Takara, Cat. No. RR820A, Japan) on a QuantStudio 5 system (Applied Biosystems, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: miR-1307-5p mediates endothelial dysfunction and inflammation induced by acute coronary syndrome by targeting PIM3.
    Article Snippet: To regulate AR TIC LE IN PR ES S miR-1307-5p and PIM3 expression in HUVECs, negative control (NC) mimics, miR-1307-5p mimics, and PIM3 overexpression (oe PIM3), NC overexpression (oe NC) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. Reverse transcription quantitative real-time PCR (RT-qPCR) Total RNA was isolated from serum and cells by means of TRIzol (Invitrogen, USA). complementary DNA was synthesized from RNA with the PrimeScript RT Kit (TaKaRa, Japan). miR-1307-5p and PIM3 levels were quantified using SYBR Green I Master Mix Reagent (Invitrogen, USA) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). miR-1307-5p/PIM3 levels were normalized using U6/ glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls and calculated by the 2 −ΔΔCt method. ..

    Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions, and cDNA was synthesized using the PrimeScript RT Kit (TaKaRa, Japan). cDNA was subsequently analyzed by RT-qPCR using 2× Universal SYBR Green Fast qPCR Mix and speci c primers (Table 1). ..

    Article Title: Mechanistic insights into 18β-glycyrrhetinic acid-induced apoptosis in SCC-9 cells revealed by TMT proteomics and network pharmacology
    Article Snippet: .. SCC-9 cells were treated with 90 μM 18β-GRA (high group) or negative control (0.05% DMSO) for 48 h. Total RNA was then extracted using TRIzol Reagent (Invitrogen, Cat. No. 15596026, USA) and reverse-transcribed into cDNA using the PrimeScript RT Kit (Takara, Cat. No. RR047A, Japan). qPCR was performed with TB Green Premix Ex Taq II (Takara, Cat. No. RR820A, Japan) on a QuantStudio 5 system (Applied Biosystems, USA). ..

    Isolation:

    Article Title: miR-1307-5p mediates endothelial dysfunction and inflammation induced by acute coronary syndrome by targeting PIM3.
    Article Snippet: To regulate AR TIC LE IN PR ES S miR-1307-5p and PIM3 expression in HUVECs, negative control (NC) mimics, miR-1307-5p mimics, and PIM3 overexpression (oe PIM3), NC overexpression (oe NC) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. Reverse transcription quantitative real-time PCR (RT-qPCR) Total RNA was isolated from serum and cells by means of TRIzol (Invitrogen, USA). complementary DNA was synthesized from RNA with the PrimeScript RT Kit (TaKaRa, Japan). miR-1307-5p and PIM3 levels were quantified using SYBR Green I Master Mix Reagent (Invitrogen, USA) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). miR-1307-5p/PIM3 levels were normalized using U6/ glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls and calculated by the 2 −ΔΔCt method. ..

    Article Title: RHOV couples EMT-associated plasticity to cytoskeletal execution of invasion and metastasis.
    Article Snippet: .. RNA was isolated using SteadyPure Quick RNA Kit (#AG21023, Agbio), and cDNA synthesized using PrimeScript RT Kit (#RR047A, Takara). qRT-PCR was performed with SYBR Green (#FP205, Tiangen) on a QuantStudioTM 6 Pro system. ..

    Synthesized:

    Article Title: miR-1307-5p mediates endothelial dysfunction and inflammation induced by acute coronary syndrome by targeting PIM3.
    Article Snippet: To regulate AR TIC LE IN PR ES S miR-1307-5p and PIM3 expression in HUVECs, negative control (NC) mimics, miR-1307-5p mimics, and PIM3 overexpression (oe PIM3), NC overexpression (oe NC) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. Reverse transcription quantitative real-time PCR (RT-qPCR) Total RNA was isolated from serum and cells by means of TRIzol (Invitrogen, USA). complementary DNA was synthesized from RNA with the PrimeScript RT Kit (TaKaRa, Japan). miR-1307-5p and PIM3 levels were quantified using SYBR Green I Master Mix Reagent (Invitrogen, USA) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). miR-1307-5p/PIM3 levels were normalized using U6/ glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls and calculated by the 2 −ΔΔCt method. ..

    Article Title: RHOV couples EMT-associated plasticity to cytoskeletal execution of invasion and metastasis.
    Article Snippet: .. RNA was isolated using SteadyPure Quick RNA Kit (#AG21023, Agbio), and cDNA synthesized using PrimeScript RT Kit (#RR047A, Takara). qRT-PCR was performed with SYBR Green (#FP205, Tiangen) on a QuantStudioTM 6 Pro system. ..

    Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions, and cDNA was synthesized using the PrimeScript RT Kit (TaKaRa, Japan). cDNA was subsequently analyzed by RT-qPCR using 2× Universal SYBR Green Fast qPCR Mix and speci c primers (Table 1). ..

    SYBR Green Assay:

    Article Title: miR-1307-5p mediates endothelial dysfunction and inflammation induced by acute coronary syndrome by targeting PIM3.
    Article Snippet: To regulate AR TIC LE IN PR ES S miR-1307-5p and PIM3 expression in HUVECs, negative control (NC) mimics, miR-1307-5p mimics, and PIM3 overexpression (oe PIM3), NC overexpression (oe NC) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. Reverse transcription quantitative real-time PCR (RT-qPCR) Total RNA was isolated from serum and cells by means of TRIzol (Invitrogen, USA). complementary DNA was synthesized from RNA with the PrimeScript RT Kit (TaKaRa, Japan). miR-1307-5p and PIM3 levels were quantified using SYBR Green I Master Mix Reagent (Invitrogen, USA) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). miR-1307-5p/PIM3 levels were normalized using U6/ glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls and calculated by the 2 −ΔΔCt method. ..

    Article Title: RHOV couples EMT-associated plasticity to cytoskeletal execution of invasion and metastasis.
    Article Snippet: .. RNA was isolated using SteadyPure Quick RNA Kit (#AG21023, Agbio), and cDNA synthesized using PrimeScript RT Kit (#RR047A, Takara). qRT-PCR was performed with SYBR Green (#FP205, Tiangen) on a QuantStudioTM 6 Pro system. ..

    Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions, and cDNA was synthesized using the PrimeScript RT Kit (TaKaRa, Japan). cDNA was subsequently analyzed by RT-qPCR using 2× Universal SYBR Green Fast qPCR Mix and speci c primers (Table 1). ..

    Quantitative RT-PCR:

    Article Title: RHOV couples EMT-associated plasticity to cytoskeletal execution of invasion and metastasis.
    Article Snippet: .. RNA was isolated using SteadyPure Quick RNA Kit (#AG21023, Agbio), and cDNA synthesized using PrimeScript RT Kit (#RR047A, Takara). qRT-PCR was performed with SYBR Green (#FP205, Tiangen) on a QuantStudioTM 6 Pro system. ..

    Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions, and cDNA was synthesized using the PrimeScript RT Kit (TaKaRa, Japan). cDNA was subsequently analyzed by RT-qPCR using 2× Universal SYBR Green Fast qPCR Mix and speci c primers (Table 1). ..

    Negative Control:

    Article Title: Mechanistic insights into 18β-glycyrrhetinic acid-induced apoptosis in SCC-9 cells revealed by TMT proteomics and network pharmacology
    Article Snippet: .. SCC-9 cells were treated with 90 μM 18β-GRA (high group) or negative control (0.05% DMSO) for 48 h. Total RNA was then extracted using TRIzol Reagent (Invitrogen, Cat. No. 15596026, USA) and reverse-transcribed into cDNA using the PrimeScript RT Kit (Takara, Cat. No. RR047A, Japan). qPCR was performed with TB Green Premix Ex Taq II (Takara, Cat. No. RR820A, Japan) on a QuantStudio 5 system (Applied Biosystems, USA). ..



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